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recombinant murine full length adiponectin  (BioVendor Instruments)


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    Structured Review

    BioVendor Instruments recombinant murine full length adiponectin
    Summary of analyte binding to <t> adiponectin. </t>
    Recombinant Murine Full Length Adiponectin, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant murine full length adiponectin/product/BioVendor Instruments
    Average 86 stars, based on 1 article reviews
    recombinant murine full length adiponectin - by Bioz Stars, 2026-03
    86/100 stars

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    1) Product Images from "Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity"

    Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20071541

    Summary of analyte binding to adiponectin.
    Figure Legend Snippet: Summary of analyte binding to adiponectin.

    Techniques Used: Binding Assay

    Adiponectin suppressed NGFβ-induced neurite outgrowth and cell swelling. ( A , B ) PC12 cells were treated with increasing concentration of NGFβ either in the presence or absence of full-length adiponectin (fADPN, 1 µg/mL) and globular adiponectin (gADPN, 1 µg/mL). Representative results of the cells (arrowhead: neurite) are shown in A and results (percentage of the cells with axon) from five independent experiments are summarized in B. ( C , D ) PC12 cells were treated with NGFβ (1 ng/mL) either in the presence or absence of full length adiponectin and globular adiponectin (0.1 and 1 g/mL). The ratio of the cell with axon ( C ) and the changes in cell body size ( D ) are determined and summarized from three independent experiments. * indicates the statistically significant difference ( p < 0.05) from NGFβ treatment alone (Cont).
    Figure Legend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth and cell swelling. ( A , B ) PC12 cells were treated with increasing concentration of NGFβ either in the presence or absence of full-length adiponectin (fADPN, 1 µg/mL) and globular adiponectin (gADPN, 1 µg/mL). Representative results of the cells (arrowhead: neurite) are shown in A and results (percentage of the cells with axon) from five independent experiments are summarized in B. ( C , D ) PC12 cells were treated with NGFβ (1 ng/mL) either in the presence or absence of full length adiponectin and globular adiponectin (0.1 and 1 g/mL). The ratio of the cell with axon ( C ) and the changes in cell body size ( D ) are determined and summarized from three independent experiments. * indicates the statistically significant difference ( p < 0.05) from NGFβ treatment alone (Cont).

    Techniques Used: Concentration Assay

    Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. ( A ) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. ( B ) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown ( n = 5). ( C – E ) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and ( C ) mRNA expression of AdipoR1 and AdipoR2 are shown. ( D ) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown ( n = 3). ( E ) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown ( n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in A and the ratio calculated was 0 as in B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference ( p < 0.05) from cont. or NGFβ treatment alone.
    Figure Legend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. ( A ) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. ( B ) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown ( n = 5). ( C – E ) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and ( C ) mRNA expression of AdipoR1 and AdipoR2 are shown. ( D ) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown ( n = 3). ( E ) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown ( n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in A and the ratio calculated was 0 as in B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference ( p < 0.05) from cont. or NGFβ treatment alone.

    Techniques Used: Activation Assay, Expressing, Transfection



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    Summary of analyte binding to adiponectin.

    Journal: International Journal of Molecular Sciences

    Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

    doi: 10.3390/ijms20071541

    Figure Lengend Snippet: Summary of analyte binding to adiponectin.

    Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

    Techniques: Binding Assay

    Adiponectin suppressed NGFβ-induced neurite outgrowth and cell swelling. ( A , B ) PC12 cells were treated with increasing concentration of NGFβ either in the presence or absence of full-length adiponectin (fADPN, 1 µg/mL) and globular adiponectin (gADPN, 1 µg/mL). Representative results of the cells (arrowhead: neurite) are shown in A and results (percentage of the cells with axon) from five independent experiments are summarized in B. ( C , D ) PC12 cells were treated with NGFβ (1 ng/mL) either in the presence or absence of full length adiponectin and globular adiponectin (0.1 and 1 g/mL). The ratio of the cell with axon ( C ) and the changes in cell body size ( D ) are determined and summarized from three independent experiments. * indicates the statistically significant difference ( p < 0.05) from NGFβ treatment alone (Cont).

    Journal: International Journal of Molecular Sciences

    Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

    doi: 10.3390/ijms20071541

    Figure Lengend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth and cell swelling. ( A , B ) PC12 cells were treated with increasing concentration of NGFβ either in the presence or absence of full-length adiponectin (fADPN, 1 µg/mL) and globular adiponectin (gADPN, 1 µg/mL). Representative results of the cells (arrowhead: neurite) are shown in A and results (percentage of the cells with axon) from five independent experiments are summarized in B. ( C , D ) PC12 cells were treated with NGFβ (1 ng/mL) either in the presence or absence of full length adiponectin and globular adiponectin (0.1 and 1 g/mL). The ratio of the cell with axon ( C ) and the changes in cell body size ( D ) are determined and summarized from three independent experiments. * indicates the statistically significant difference ( p < 0.05) from NGFβ treatment alone (Cont).

    Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

    Techniques: Concentration Assay

    Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. ( A ) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. ( B ) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown ( n = 5). ( C – E ) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and ( C ) mRNA expression of AdipoR1 and AdipoR2 are shown. ( D ) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown ( n = 3). ( E ) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown ( n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in A and the ratio calculated was 0 as in B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference ( p < 0.05) from cont. or NGFβ treatment alone.

    Journal: International Journal of Molecular Sciences

    Article Title: Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

    doi: 10.3390/ijms20071541

    Figure Lengend Snippet: Adiponectin suppressed NGFβ-induced neurite outgrowth independently of its receptor activation. ( A ) Expression of AdipoR1 and AdipoR2 mRNA in the rat skeletal muscle (SM), liver and PC12 cells are shown. ( B ) PC12 cells were treated with full length adiponectin or globular adiponectin and the amounts of phosphorylated and total AMPK were determined. Representative results and the ratio of phosphorylated and total AMPK are shown ( n = 5). ( C – E ) PC12 cells were treated with unrelated (un), AdipoR1, AdipoR2 and R1 plus R2 siRNA and ( C ) mRNA expression of AdipoR1 and AdipoR2 are shown. ( D ) The transfected cells were treated with vehicle (cont.), globular adiponectin (1 µg/mL) and full length adiponectin (1 µg/mL) and the state of AMPK activation are shown ( n = 3). ( E ) The transfected cells were treated with vehicle (cont.), NGFβ (1 ng/mL) or NGFβ plus full length adiponectin (1 µg/mL) and the ratios of the cell with axon are shown ( n = 3). The transfected cells treated with vehicle did not induce any neurite (axon) as shown in A and the ratio calculated was 0 as in B. Thus, bar for control value of each siRNA was not seen. * indicates the statistically significant difference ( p < 0.05) from cont. or NGFβ treatment alone.

    Article Snippet: Recombinant murine full-length adiponectin was purchased from Biovender Laboratory Medicine, Inc. (Bmo, Czech Republic), while recombinant murine globular adiponectin and recombinant human PDGF-BB were purchased from Wako Pure Chemical (Osaka, Japan).

    Techniques: Activation Assay, Expressing, Transfection

    Table 1

    Journal: The Journal of Physiology

    Article Title: Chronic maternal infusion of full-length adiponectin in pregnant mice down-regulates placental amino acid transporter activity and expression and decreases fetal growth

    doi: 10.1113/jphysiol.2011.226399

    Figure Lengend Snippet: Table 1

    Article Snippet: Pumps contained 0.75 μg μl −1 (in phosphate-buffered saline (PBS)) of full-length murine recombinant adiponectin (Alexis Biochemicals, San Diego, CA, USA), giving an infusion rate of 0.62 ± 0.02 μg (g body weight) −1 day −1 (fADN-infused, n = 7) or vehicle only (Control, n = 9).

    Techniques: